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I can recommend to review this on the datasheet for further information. Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 2: Wild-type HAP1 cell lysate Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 4: Caspase-3 knockout HAP1 cell lysate Lanes 1 - 4: Merged signal (red and green). Green - ab184787 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa. ab184787 was shown to recognise pro Caspase 3 ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature.

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ab13847; Abcam),  28 Dec 2020 Western blot was performed as previously descri- bed22. The blots were incubated with antibodies against cleaved-caspase-3 (ab13847, Abcam,  ThermoFisher. Scientific. Rabbit anti-mouse Ki-67 ab15580.

Tystnad Av MiR-193a-5p ökar Kemosensitiviteten Hos

.. Cy3 conjugated secondary antibody (anti-mouse, 1:800) or 488-conjugated secondary antibody (anti-rabbit, 1:800) was incubated with the brain cryosection for 2 h at room temperature. 2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 and -BAX (Ab3154) were purchased from Abcam (Cambridge, UK). An anti-active caspase-3 specific antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting. Protein AG agarose was purchased from Pierce (Waltham, MA, USA).

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2020-05-18 · Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 and -BAX (Ab3154) were purchased from Abcam (Cambridge, UK). An anti-active caspase-3 specific antibody was purchased from Abcam (Ab13847) to detect the cleaved 17 kD form of caspase-3 by Western blotting. Protein AG agarose was purchased from Pierce (Waltham, MA, USA). HEK293 (RRID:CVCL_0045) and 769-P (RRID:CVCL_1050) kidney 2020-12-01 · Briefly, the specimen was fixed in 10% formaldehyde and embedded in paraffin. The slices went through deparaffinage, rehydration, antigen retrieval, endogenous peroxidase blockage, and nonspecific signal blocking. Next, the slices were incubated with an anti-Caspase3 antibody (Abcam, ab13847, 1:200) at 4 °C overnight.

The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/mL, applied to tissues for 15 minutes, and detected with Abcam Steady DAB/Plus (ab103723) for 5 minutes. 1:1000), p-Akt (Abcam, ab38449, 1:1000), Akt(Abcam, ab8805, 1:1000) overnight at 4 C. Afterward, we washed the membranes with TBST and incubated them with horseradish 2021-01-12 · Mutations of WD40 repeat domain 60 (WDR60) have been identified in short-rib polydactyly syndromes (SRPS I–V), a group of lethal congenital disorders characterized by short ribs, polydactyly Tunicamycin induces NSCLCs apoptosis 5311 Int J Clin Exp Med 2018;11(5):5310-5322 many tumor cells [13]. However, the potential mechanisms of Tunicamycin-induced apoptosis ab13847), cleaved caspase 3 (Abcam, ab2302), caspase 9 and cleaved caspase 9 (Abcam, ab184786), IκBα (Cell Signaling Technology, #4814), phosphor‐SPAK/JNK (Cell Signaling Technology, #9255), 2019-02-01 · 1.
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I can recommend to review this on the datasheet for further information. Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. Abcam caspase-3 antibody (Abcam, ab13847) was used in immunocytochemistry on human samples at 1:100 (fig s9) and in western blot on human samples at 1:1000 (fig s9). Nat Commun (2016) ncbi domestic rabbit polyclonal active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847 IHC (PFA fixed), WB RMk, Xl, Zfsh active Caspase 3 M WB Rb Hu ab77973 Caspase3 Abcam ab13847 mmp-2 Abcam ab37150 mmp-9 Abcam ab38898 Table S5. Specific siRNAs for depletion or overexpression of GAS5, YBX1, and miR-21, and nonspecific Abcam Ab13847; RRID: AB_443014 Anti-Caspase 3 (Rabbit polyclonal) Cell Signaling Technology #9662S; RRID: AB_10694681 Slides were stained to detect the expression of PRDX1 (abcam; Anti-PRDX1 antibody ab41906), γ-H2A.X (abcam; Anti-γ-H2A.X phosphorylated S139 antibody; ab2893), Ki 67 (abcam; Anti Ki67 antibody; ab 15580), 53BP1 (abcam; Anti-53BP1 antibody; ab21083) and cleaved caspase 3 (abcam; Anti-cleaved caspase 3 antibody; ab13847) using an HRP/DAB domestic rabbit monoclonal (E61) reactivity: human conjugate: HRP application: western blot, immunohistochemistry - paraffin section The sections were incubated with primary antibodies anti-MVH (ab13840, Abcam), LC3B (Ab51520, Abcam), CASP9 (Ab202068, Abcam), CASP3 (Ab13847, Abcam), and Stat3 (9139p, Cell signaling technology Hong X, O Donnell J, Salazar C, Van Brocklyn J, Barnett K, Pearl D, et al.The selective Aurora-A kinase inhibitor MLN8237 (alisertib) potently inhibits proliferation of glioblastoma neurosphere tumor stem-like cells and potentiates the effects of temozolomide and ionizing radiation. Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active).

active Caspase 3 human, reacts with human, mouse, rat, fruit fly (drosophila melanogaster), indian muntjac, rhesus monkey, xenopus laevis and zebrafish (pmid 19823566)predicted to react with chinese hamster (100 identity with immunogen), dog (100 identity with immunogen) and pig (100 identity with immunogen) due to sequence homology 6044 | CHE E T AL. up-regulation of several osteogenic genes, including runt-related tran-scription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin.5 caspase3 (ab49822, Abcam), total caspase3 (ab13847, Abcam), E-cadherin (CST, Danvers, MA, USA) and β-actin (cat. no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C. Sec-ondary antibodies including goat anti-rabbit (ab205718, Abcam) and goat anti-mouse (ab6789, Abcam) were 2020-01-30 · The membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 2 h at room temperature, and then incubated overnight at 4 °C with primary antibodies (Abcam, Shanghai China) against B-cell lymphoma-2 (Bcl-2, ab32124), Bcl-2-associated X (Bax, ab32503), total poly (ADP-ribose) polymerase (PARP, ab74290), X-linked inhibitor of apoptosis (XIAP, ab21278 2018-04-03 · Slides were placed on the Bond Max autostainer for staining with the Abcam antibody for Active Caspase 3 (Rabbit polyclonal, ab13847). The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/mL, applied to tissues for 15 minutes, and detected with Abcam Steady DAB/Plus (ab103723) for 5 minutes. 1:1000), p-Akt (Abcam, ab38449, 1:1000), Akt(Abcam, ab8805, 1:1000) overnight at 4 C. Afterward, we washed the membranes with TBST and incubated them with horseradish 2021-01-12 · Mutations of WD40 repeat domain 60 (WDR60) have been identified in short-rib polydactyly syndromes (SRPS I–V), a group of lethal congenital disorders characterized by short ribs, polydactyly Tunicamycin induces NSCLCs apoptosis 5311 Int J Clin Exp Med 2018;11(5):5310-5322 many tumor cells [13]. However, the potential mechanisms of Tunicamycin-induced apoptosis ab13847), cleaved caspase 3 (Abcam, ab2302), caspase 9 and cleaved caspase 9 (Abcam, ab184786), IκBα (Cell Signaling Technology, #4814), phosphor‐SPAK/JNK (Cell Signaling Technology, #9255), 2019-02-01 · 1.
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ab13847 and ab8245 (loading control to GAPDH) were diluted to 1/500 and 1/10000 respectively and incubated overnight at 4°C. Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN. UniProt Code History.

Primary Cell Isolation, Culture, and Cell  1: 1000), Bax (Abcam, ab32503, 1:1000), Bcl2(Abcam, ab32124, 1:1000), Caspase3(Abcam, ab13847, 1:1000),. E-cadherin (Abcam, ab40772, 1:1000),  30 Sep 2020 human ASIC1a (1 μg/mL, ab94753, Abcam, p-PI3K (1:1,000, ab182651, Abcam, UK), AKT UK), caspase-3 (1:500, ab13847, Abcam, UK). 19 Apr 2018 Primary antibodies included anti-Bcl-2 (ab59348, 1:1000; Abcam, UK), 1:500; Abcam), anti-cleaved-caspase-3 (ab13847, 1:500; Abcam),  3 Dec 2020 sc-7382, Santa Cruz Biotechnology), caspase-3 (No. ab13847, Abcam, Cambridge, MA, USA), cleaved caspase3 (No. ab2302, Abcam), caspase  Cyt c rabbit antibody, ab18738, caspase-3 rabbit antibody, ab13847, Abcam, USA; β-actin rabbit antibody, bs-0061R, bioss, Beijing; goat anti-rabbit IgG (H+L),   cam, ab108319, Cambridge, UK) and caspase-3 levels. (Abcam, ab13847, Cambridge, UK) were assessed im- munohistochemically in other brain hemispheres  15 Nov 2018 ab15580); anti-Caspase3 (1:500, Abcam ab13847). GFAP-positive as- trocytes were not quantified following detachment and injection, be-. 23 Apr 2018 following primary antibodies: NeuN (Millipore, MAB377, 1:300), Epac2 (1:200, Cell Signaling, 43239), caspase-3 (1:200, Abcam, ab13847).
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Tystnad Av MiR-193a-5p ökar Kemosensitiviteten Hos

no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C. Sec-ondary antibodies including goat anti-rabbit (ab205718, Abcam) and goat anti-mouse (ab6789, Abcam) were 2020-01-30 · The membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 2 h at room temperature, and then incubated overnight at 4 °C with primary antibodies (Abcam, Shanghai China) against B-cell lymphoma-2 (Bcl-2, ab32124), Bcl-2-associated X (Bax, ab32503), total poly (ADP-ribose) polymerase (PARP, ab74290), X-linked inhibitor of apoptosis (XIAP, ab21278 2018-04-03 · Slides were placed on the Bond Max autostainer for staining with the Abcam antibody for Active Caspase 3 (Rabbit polyclonal, ab13847). The Active Caspase 3 antibody was conjugated to HRP complex using an Abcam HRP Conjugation Kit (ab102890), diluted to approximately 3µg/mL, applied to tissues for 15 minutes, and detected with Abcam Steady DAB/Plus (ab103723) for 5 minutes. 1:1000), p-Akt (Abcam, ab38449, 1:1000), Akt(Abcam, ab8805, 1:1000) overnight at 4 C. Afterward, we washed the membranes with TBST and incubated them with horseradish 2021-01-12 · Mutations of WD40 repeat domain 60 (WDR60) have been identified in short-rib polydactyly syndromes (SRPS I–V), a group of lethal congenital disorders characterized by short ribs, polydactyly Tunicamycin induces NSCLCs apoptosis 5311 Int J Clin Exp Med 2018;11(5):5310-5322 many tumor cells [13]. However, the potential mechanisms of Tunicamycin-induced apoptosis ab13847), cleaved caspase 3 (Abcam, ab2302), caspase 9 and cleaved caspase 9 (Abcam, ab184786), IκBα (Cell Signaling Technology, #4814), phosphor‐SPAK/JNK (Cell Signaling Technology, #9255), 2019-02-01 · 1.